In this report, we describe the development and application of a semi-automated method for integrating mechanistic information from adverse outcome pathways (AOPs) into the Biomedical Data Translator (“Translator”) ecosystem (Biomedical Data Translator Consortium, 2018; Fecho, Thessen, et al., 2022) as causal activity models (CAMs). These efforts provide a foundation for integrating AOPs and information on associated chemical safety regulatory endpoints and methods of measurement into the biomedical observations already within the Translator knowledge graph.